Xusheng Ai is a Graduate Research Assistant at The University of Texas MD Anderson Cancer Center. He designs computational frameworks for ultra-sparse single-cell epigenomic data, with an emphasis on scCUT&Tag profiles from CAR-T cells. By converting these data into high-resolution tile-by-cell matrices and applying advanced statistical and machine-learning techniques, he reveals biologically meaningful cell clusters. His work links chromatin patterns to CAR-T cell phenotypes, deepening our understanding of cancer biology and guiding the discovery of new therapeutic targets.